Function of a mysterious component of the inner ear revealed a yearning for publius top universities in new york

Recently I stumbled across another example while in the waiting room of the audiology department at the VA hospital. A copy of the magazine “ hearing health” was sitting beside me and I started thumbing through it, and found summaries of some amazing research happening in the medical field of hearing.

It’s difficult for me to understand why darwinists continue to insist that such ‘functionally intentional’ machines in our bodies somehow came into existence through random mutations over ‘deep time.’ ‘functionally intentional’ machines are all around us and within us, and in great abundance. What are your thoughts on this?

A few years ago, ian swinburne, HMS research fellow in systems biology, noticed something odd while conducting a time-lapse microscopy study of the inner ear of zebrafish. A tiny structure in the inner ear was pulsing like clockwork, inflating and deflating over and over.

As swinburne and his postdoctoral advisor sean megason, HMS associate professor of systems biology, probed further, they discovered that the structure was the endolymphatic sac, a fluid-filled pocket connected to the rest of the inner ear by a long, thin duct.

“scientists have known about the existence of the endolymphatic sac for maybe 300 years, but it wasn’t understood exactly what it does,” megason said. “it’s even often missing in models or textbook cartoons of the inner ear. We didn’t set out to study it, but we became interested once we saw its striking behavior.”

Over the course of the next several years, swinburne and megason worked to better understand the function of this mysterious structure. Uni in to do so, they had to visualize it in action. Collaborating with some of the world’s leading microscopy laboratories, they pieced together different views of the endolymphatic sac until a clear picture emerged.

In a study published june 19 in elife, the team reported the results of their investigation: the endolymphatic sac acts as a pressure-relief valve and is formed by a thin barrier of cellular projections that opens and closes to regulate the release of fluid from inside the inner ear.

Their findings reveal a unique biological mechanism for maintaining fluid pressure and composition and may inform the study and treatment of disorders involving defects in inner ear pressure such as meniere’s disease, a condition marked by vertigo, hearing loss and ringing in the ears. The results could also help researchers study pressure control in other organs such as the eyes and kidneys, which also have liquid-filled cavities.

The inner ear is the sensory organ responsible for hearing and balance and is composed of several complex structures. In mammals, sound is detected by the snail shell-shaped cochlea, and head movement is detected by three hollow loops of bone called the semicircular canals.

All the structures of the inner ear are interconnected and filled with specialized fluid, which moves in response to sound waves or head movement. These subtle fluid movements are detected by sensory cells and converted into neural signals for the brain to process. Washington university act scores both the pressure and chemical composition of inner ear fluid must be carefully maintained, and certain disorders such as ménière’s disease are thought to stem from abnormal pressure fluctuations.

The inner ears of zebrafish embryos, which swinburne and megason study, are much more visible. When the team first observed the pulsing behavior of the endolymphatic sac, they suspected a connection with pressure control. Proving it, however, was another matter.

“we had all these movies where you could see the whole structure pulsing, and when ian injected dye into the sac we could see fluid flowing out,” megason said. “but it wasn’t clear how that fluid was getting out. It seemed like something weird was going on.”

At that time, swinburne was also engaged in a side project reviewing previously published studies of various zebrafish with mutated genes. One of the mutants he stumbled upon, with an abnormal form of the transcription factor lmx1bb, had endolymphatic sacs that were far larger than normal.

Through dye injection experiments, they discovered that in lmx1bb mutants, inner ear fluid was not flowing out of the endolymphatic sac as it should, and the buildup of fluid caused the structure to balloon. The team noted that normally, a little bit of fluid also leaks back into the sac when it deflates. No such leakage occurred in lmx1bb mutants, however, suggesting that the structure was somehow closed.

They were stumped until they connected with jeff lichtman, the jeremy R. Knowles professor of molecular and cellular biology and the santiago ramón y cajal professor of arts and sciences at harvard university. The lichtman lab specializes in imaging the brain, and among the data they’ve collected happened to be high-resolution electron micrographs of the inner ear.

The team’s analyses revealed that normal endolymphatic sacs contain an extremely thin shell of these overlapping lamellae, which they termed “lamellar barriers.” in most tissues, cells are tightly connected and water cannot pass between them. In the endolymphatic sac, however, cells appeared to have small gaps between them, which are covered by lamellar barriers.

To further investigate, the researchers teamed up with microscopy pioneer and nobel laureate eric betzig of the howard hughes medical institute and tomas kirchhausen, HMS professor of cell biology and a professor of pediatrics at boston children’s hospital.

Earlier this year, betzig, kirchhausen, megason and colleagues published a seminal paper describing a new technology called adaptive optics-lattice light sheet microscopy, which allows researchers to capture 3D images and movies of cells inside living organisms in unprecedented detail.

Their results implicate the endolymphatic sac as a pressure-relief valve for the inner ear, but many mysteries remain for future study, such as how the lamellar barriers connect with each other, whether they are opened by physical pressure or some pressure-sensing protein and whether this same mechanism is present in other animals such as mice and humans.

The team also suspects that this mechanism may be present in other organs, such as the eye, brain and kidneys, which also contain pressurized fluid-filled cavities. American university acceptance rate of particular interest is the role of genes related to lmx1bb, which, when mutated in mice, cause kidney and eye problems.

Mutations to lmx1 genes in humans have been linked to glaucoma, a condition where fluid builds up in the front part of the eye. A better understanding of lamellar barriers and pressure- relief mechanisms could help inform the study and treatment of these diseases, the authors suggest.

“this study was definitely a case of seeing is believing,” megason said. “it was very important to have cutting-edge microscopy on many different fronts. Each of these different microscope techniques gave us a different piece of the puzzle and when put together, we get the whole picture.”

• ian A swinburne, kishore R mosaliganti, srigokul upadhyayula, tsung-li liu, david G C hildebrand, tony Y -C tsai, anzhi chen, ebaa al-obeidi, anna K fass, samir malhotra, florian engert, jeff W lichtman, tomas kirchausen, eric betzig, sean G megason. Lamellar projections in the endolymphatic sac act as a relief valve to regulate inner ear pressure. ELife, 2018; 7 DOI: 10.7554/elife.37131

Harvard medical school. (2018, june 28). Function of a mysterious component of the inner ear revealed: endolymphatic sac acts as a pressure release valve for the inner ear. ScienceDaily. Retrieved october 16, 2018 from http://www.Sciencedaily.Com/releases/2018/06/180628151928.Htm

Harvard medical school. “function of a mysterious component of the inner ear revealed: endolymphatic sac acts as a pressure release valve for the inner ear.” sciencedaily. Http://www.Sciencedaily.Com/releases/2018/06/180628151928.Htm (accessed october 16, 2018).